AAV パッケージング – 研究グレード
- プラスミド抽出からウイルスまで最短12日で対応
- 高純度、低エンドトキシン、低エンプティシェル率
- 70種類以上のセロタイプと50,000件以上のプロジェクトで培った専門的サポート
主な利点
-
迅速な納品
AAV 5E+13GCの場合、12～15営業日 -
保証力価
≥1E+13GC/mL（qPCRゲノムコピー数/ml） -
高純度
SDS-PAGEで分析 -
高収率
最大1E+16GC -
低エンドトキシン
<10EU/ml、生体内実験に適しています -
低エンドトキシン
<30% -
複数の血清型
70種類以上の血清型オプションをご用意 -
豊富な経験
10,000件以上のカスタムAAVプロジェクトを納品 -
経験豊富なテクニカルサポート
長年のAAV経験を持つ博士レベルのチーム
サービスの詳細
Price and Turnaround
*The indicated titers are guaranteed except when the insert exceeds the packaging capacity (4.7 kb) or if you choose to provide us with your own modified rep/cap plasmid or helper plasmid.
| AAV Packaging Serotypes | Guarantoed Yield (GC)* | Lead Time (Business Days) |
|---|---|---|
| Normal-yield AAV Serotypes | 2E+12 GC | 12-15 Days |
| 5E+12 GC | ||
| 1E+13 GC | ||
| 2E+13 GC | ||
| 5E+13 GC | ||
| 1E+14 GC | ||
| 2E+14 GC | 18-24 Days | |
| 5E+14 GC | ||
| 1E+15 GC | 30-45 Days | |
| 2E+15 GC | ||
| Low-yield AAV Serotypes (AAV4, 6, etc.) | 2E+12 GC | 12-15 Days |
| 5E+12 GC | ||
| 1E+13 GC | ||
| 2E+13 GC | ||
| 5E+13 GC | ||
| 1E+14 GC | 18-24 Days | |
| 2E+14 GC | ||
| 4E+14 GC |
- GC = Genome copies.
- For these extremely low-yield AAV serotypes without production data, we are not able to guarantee the final yield or titer specified here.
- If you would like to use any AAV serotypes that are currently under patent, and your application is for commercial use, we advise that you contact the patent owner to obtain authorization beforehand.
Storage Requirements:
- Store the virus at -80°C, and place it on ice during operation.
- Calculate your expected usage in advance and PackGene will aliquot your virus according to your pre-determined requirements. This can help avoid unnecessary thawing and re-freezing after receiving your AAVs since freeze thaw cycles influence virus viability. If aliquoting is required, it is recommended to use PCR tubes with siliconized inner walls, or special virus preservation tubes with low protein binding rates.
- Thaw your virus aliquots in an ice bath immediately before use.
- Dilute with PBS or PBS / 0.001% F-68 if needed.
Quality Control
A variety of AAV-based QC assays have been developed by PackGene’s experienced QC team. QC tests are aimed at verification of the identity, purity, and potency of AAV viral particles for both in vitro and in vivo studies. AAV genome copies are quantified via SYBR qPCR with ATCC’s Reference AAV for titer calibration. Purity is determined by Coomassie-Blue staining.
We guarantee the endotoxin level of the AAV particles lower than 10 EU/ml. We also offer additional QC tests including ddPCR , TEM, TCID50 tittering and other QC services. Please check AAV Analytical Services to learn more.
| Category | QC Assays | QC Standard |
|---|---|---|
| Identity | Identity – GOI Sequence | Additional QC |
| Purity | SDS-PAGE Coomassie Blue Staining | Free QC |
| TEM | Additional QC | |
| AUC | Additional QC | |
| Potency & Content | qPCR | Free QC |
| ddPCR | Additional QC | |
| TCID50 | Additional QC | |
| Capsid Titer-ELISA | Additional QC | |
| Impurity | Endotoxin Test | Free QC |
| Mycoplasma Detection | Additional QC | |
| Sterility Test | Additional QC | |
| Residual Plasmid Test | Additional QC |
パフォーマンス
-
Standard QC
- Restriction digestion analysis using multiple endonuclease to verify the plasmids to be used for AAV packaging.
- AAV Titering by qPCR (SYBR Green with standard curve for quantification)
Note: ATCC VR-1816™ was used as the standards for AAV qPCR titering.- Endotoxin Test by LAL assay
- AAV purity analysis by SDS-PAGE and Coomassie Staining (silver staining available upon request)
Legend: Lanes 2-5 and 7-1: AAV samples produced at PackGene. Lane 1,6: Marker -
Other Available Analytical Tests
