レンチウイルスパッケージングサービス
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最短8〜12営業日での納品
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トランスダクション後qPCRによる正確な力価測定
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専門的サポートと安全な配送を備えたワンストップかつ安全なソリューション
主な利点
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レンチウイルスパッケージングサービス
配送時間は最短8～12営業日 -
正確な力価測定
導入後のqPCRにより、過大評価の懸念が排除されます。 -
プロフェッショナルな技術サポート
お問い合わせやご要望への回答は1営業日以内にお届けします。 -
強化された安全性
「自己不活性化」第3世代システムによりバイオセーフティが向上 -
ワンストップソリューション
ベクター設計とウイルスパッケージングから徹底的な分析とテストまで -
安全な配送
最適な効率を維持するためのドライアイスとコールドチェーン物流
サービスの詳細
| In vitro grade | In vivo grade | |
|---|---|---|
| Application | Cell culture | In vivo study |
| Quantity | 5E7 TU and up | 5E7 TU and up |
| Functional titer (post-transduction qPCR) |
≥1E+8TU/mL | ≥1E+8TU/mL |
| Physical titer (p24 or qPCR) |
≥ 2E+9 GC/mL ≥ 1E+10 LP/mL |
≥ 2E+9 GC/mL ≥ 1E+10 LP/mL |
| Purification | Crude | Sucrose gradient |
| QC (see add-on QC below) |
Post-transduction qPCR, fluorescence images if contain fluorescence | Post-transduction qPCR, fluorescence images if contain fluorescence |
| Timeline | Start from 7 business days | 3-4 weeks |
- Lentivirus will be offer at a concentration of >1E8 TU/mL
- Yield is not guaranteed for constructs with elements detrimental to the viral packaging process. This includes but is not limited to, toxic genes, genes that disrupt viral packaging, genes that disrupt cell or lentiviral RNA integrity, sequences prone to rearrangement or secondary structures, nucleoproteins, transmembrane proteins, receptor genes, and gene segments with over 6.5kb between LTRs.
Quality Control
| Category | QC test | Description | Release standard |
|---|---|---|---|
| Titer | qPCR | qPCR quantifies the number of viral genome copies providing an accurate measure of the viral titer | Report titer |
| Post-transduction qPCR | Infection of cells followed by quantitative qPCR to determine the titer of lentiviruses | Report transduction unit | |
| Transduction test | Gradient dilution of infected cells and count of fluorescent cells | Provide bright field and fluorescent microscopy images | |
| p24 Elisa* | ELISA test for the p24 protein, a core capsid protein of HIV that is used to measure lentivirus titer | Report titer | |
| Contamination | Mycoplasma test* | Measure mycoplasma DNA by PCR | Guaranteed negative |
| Bioburden* | Plate count method: Quantification of live microorganisms (fungi, bacteria, etc.) using colony-forming units (CFUs) | No growth on the agarose plate | |
| Safety | Endotoxin LAL* | Measure endotoxin level by gel clot assay | Report |
* QC with additional charges
Need control lentivirus?
| Off-the-shelf lentivirus | Virus per vial | Titer |
|---|---|---|
| CAG.EGFP.T2A.Puro.WPRE | 2E7 TU | ≥1E+8TU/mL |
| CAG.EGFP.T2A.Bsd.WPRE | ||
| CAG.EGFP.T2A.Hygro.WPRE | ||
| CAG.mCherry.T2A.Puro.WPRE | ||
| CAG.mCherry.T2A.Bsd.WPRE | ||
| CAG.mCherry.T2A.Hygro.WPRE | ||
| EF1.SpCas9.T2A.Puro.WPRE | ||
| EF1.SpCas9.T2A.EGFP.WPRE | ||
| U6.NCshRNA.CAG.EGFP.T2A.Puro.WPRE | ||
| U6.NCshRNA.CAG.mCherry.T2A.Bsd.WPRE |
パフォーマンス
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Post-Transduction Titer Ensure batch-to-batch consistency
No more concerns of overestimation
Accurate titer measurements by post-transduction qPCR
96-well plate, 1.5E+04 cells/well, virus 1.8µL, 72hrs after transduction See more QC test data -
How we measure Post-Transduction Titer
